AUTOMATED PLANT EXTRACTION METHOD AND ARRAYS FOR HIGH THROUGHPUT SCREENING

摘要

An automated and validated method for preparing plant extracts for use in the high throughput screening of biological assays uses a robotic sample processor for the aspiration and dispensing of solvent extracts and a fully automated solid phase extraction apparatus to produce column fractionated plant extracts. The method comprises the steps of: (a) thoroughly extracting a biological source material with at least one solvent to produce at least one solvent extract containing the potentially bio-active biochemicals of the source material; (b) removing from the solvent extracts interferences, comprising non-selectively bio-active compounds that interfere with biological assays; (c) subjecting the solvent extracts to chromatography by an automated system for handling samples; (d) detecting the potentially selectively bio-active biochemicals of the source material in the eluent; and (e) collecting the biochemicals in isolated fractions having standardized concentrations under control by the automated system with associated data.