| 摘要 |
The invention relates to the composition, production and use of luminescent microparticles and nanoparticles. These particles can either be used as internal standards for referencing fluorescence signals, or as markers for marking and detecting biomolecules. Luminescence dyes are incorporated, in inert form, into solid materials such that they are protected from the influence of chemical and biological compounds in aqueous sample constituents. In this incorporated form, the photophysical characteristics of the dyes (spectral characteristic, luminescence quantum efficiency, luminescence fading time and polarization) remain unaffected by sample parameters that vary. Compact inorganic materials or organic polymers are selected, in particular, as an incorporating matrix which, due to their structure, do not receive biomolecules, small neutral molecules as well as ionic compounds. In particular, the interfering influence of molecular oxygen, of an efficient luminescence quencher, on luminescence measurements is eliminated in this manner. The surface of the nanoparticles and microparticles can be provided with reactive surfaces in order to enable covalent coupling of biochemicals or to eliminate the aggregation of the particles. |
