发明名称 |
TECHNIQUES FOR GROWTH AND DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS |
摘要 |
This disclosure provides an improved system for culturing human pluripotent stem (pPS) cells in the absence of feeder cells. The role of the feeder cell s can be replaced by supporting the culture on an extracellular matrix, and culturing the cells in a conditioned medium. Permanent cell lines are provid ed that can produce conditioned medium on a commercial scale. Methods have also been discovered to genetically alter pPS cells by introducing the cells with a viral vector or DNA/lipid complex. The system described in this disclosure allows for bulk proliferation of pPS cells for use in studying the biology o f pPS cell differentiation, and the production of important products for use i n human therapy.
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申请公布号 |
CA2388811(A1) |
申请公布日期 |
2001.07.19 |
申请号 |
CA20012388811 |
申请日期 |
2001.01.10 |
申请人 |
GERON CORPORATION |
发明人 |
XU, CHUNHUI;INOKUMA, MARGARET S.;GOLD, JOSEPH D.;FUNK, WALTER D.;CARPENTER, MELISSA K. |
分类号 |
C12N15/09;A61K35/12;C12N5/02;C12N5/07;C12N5/073;C12N5/0735;C12N5/077;C12N5/079;C12N5/0793;C12N5/10;C12N15/10;C12P21/02;C12Q1/02;C12R1/91;(IPC1-7):C12N5/08;C12P19/34;C12N15/63;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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