发明名称 |
Recombinant adenovirus and adeno-associated virus, cell lines, and methods of production and use thereof |
摘要 |
An adenovirus E1/E4 expressing packaging cell line is provided, which permits the generation of recombinant adenoviruses deleted in both gene regions. A method for enhancing the efficiency of transduction of a recombinant AAV into a target cell is provided by infecting a target cell with a recombinant AAV comprising a selected transgene under the control of regulatory sequences. The infected cell is contacted with an agent which facilitates the conversion of single stranded recombinant virus to its double stranded form.
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申请公布号 |
US6261551(B1) |
申请公布日期 |
2001.07.17 |
申请号 |
US19970973334 |
申请日期 |
1997.12.05 |
申请人 |
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA |
发明人 |
WILSON JAMES M.;FISHER KRISHNA J.;GAO GUANG-PING |
分类号 |
C12N15/09;A61K35/76;A61K48/00;C12N5/08;C12N5/10;C12N7/00;C12N15/35;C12N15/864;(IPC1-7):A61K48/00;C12N15/861;C12N15/63 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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