发明名称 |
METHOD FOR DETECTING VIROID AND CAPTURE PROBE USED FOR DETECTION OF VIROID |
摘要 |
PROBLEM TO BE SOLVED: To provide a method for detecting viroid, capable of stably detecting viroid in a high sensitivity by a simple operation without spending time and effort and provide capture probe used for detection of viroid. SOLUTION: This method for detecting viroid comprises a step for extracting RNA from a plant which becomes a specimen, a step for recovering only viroid RNA from the extracted RNA by using capture probe with a base sequence having complementarity with base sequence site which is common to well-known plural viroid RNAs and a step for amplifying gene by gene amplification method after reversely transferring the recovered viroid RNA to cDNA.
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申请公布号 |
JP2001190300(A) |
申请公布日期 |
2001.07.17 |
申请号 |
JP20000010011 |
申请日期 |
2000.01.13 |
申请人 |
WAKAYAMAKEN NOGYO KYODO KUMIAI RENGOKAI |
发明人 |
SANO TERUO;HIRATA YUKIMASA;YAMASHITA HIROKO;SHIBA KOUSUKE |
分类号 |
C12N15/09;C12Q1/68;C12Q1/70;C12R1/91;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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