Nucleic acid encoding delta6-acetylenase or desaturase, useful for producing plant oils with increased content of unsaturated fatty acids

摘要

Isolated nucleic acid (I) encoding polypeptides (II) with DELTA 6-acetylenase and/or DELTA 6-desaturase activity, is new. Isolated nucleic acid (I) encoding polypeptides (II) with DELTA 6-acetylenase and/or DELTA 6-desaturase activity, is new. (I) is a 2040 bp (N1), 1467 bp (N2) or 2160 bp (N3) sequence (all given in the specification), is equivalent to N1-N3 within the degeneracy of the genetic code or is a derivative of N1-N3 encoding a polypeptide that has /- 75% amino acid (aa) homology with a 483 aa sequence (given in the specification). Independent claims are also included for the following: (a) amino acid sequences encoded by (I); (b) an expression cassette (EC) containing (I) linked to one or more regulatory sequences; (c) a vector containing (I) and EC; (d) organisms containing (I), EC or the vectors of (c); (e) preparation of unsaturated fatty acids (A) or triglycerides (TG) with increased content of (A) by introducing (I) or EC into an oil-producing organism; (f) proteins (IIa) of 172 aa or 178 aa (given in the specification); (g) production of (A) or TG by using (Ia); and (h) (A) and TG produced by method (g).