Method for determining the detection value of a target RNA

摘要

The present invention provides a method of detecting RNA, comprising: (a) preparing cDNA to be measured and standard cDNA by reverse transcription of RNA as the subject of detection and standard RNA respectively; (b) adding different adapters respectively to the standard cDNA prepared at stepwise concentrations; (c) adding an adapter other than said adapters to the cDNA to be measured; (d) mixing and amplifying the adapter-tagged cDNAs obtained in the steps (b) and (c); (e) measuring the amount ratio of the cDNA measured to the standard cDNA; and (f) detecting said RNA from the measurement results.