Novel nucleotide sequences coding for the gpm gene.

摘要

Isolated polynucleotide (I), from coryneform bacteria, is: (i) at least 70% identical with a sequence (S1) that encodes a 248 amino acid (aa) sequence (II); (ii) encodes a polypeptide containing a sequence at least 70% identical with (II); (iii) complementary to (i) or (ii); or (iv) contains at least 15 consecutive nucleotides (nt) of (i)-(iii). Independent claims are also included for the following: (1) vector (III) containing (I); (2) coryneform bacterium containing (III); and (3) production of L-aa by fermenting a coryneform bacterium in which at least the phosphoglycerate mutase-encoding gene (gpm) is amplified, especially overexpressed.