发明名称 |
Methods and compositions for the detection of Candida spp. |
摘要 |
The present invention provides a rapid method for identifying species of Candida. Identification is through the use of a non-conserved regions of the ITS2 region flanked by highly conserved functional domains. Detection of members of the Candida and Aspergillus genera is enhanced by the detection of genus-specific regions of the 5.8S rRNA gene. The present invention provides isolated nucleic acids that selectively hybridize to the fungal genus-specific 5.8S rRNA region, and to the species-specific ITS2 region. The invention provides for nucleic acid sequences for use as selective probes for fungal genus-specific probes and as Candida species-specific probes. The present invention provides methods for the use of the Candida species-specific probes that allow the detection and identification of the individual species in biological samples.
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申请公布号 |
US6235890(B1) |
申请公布日期 |
2001.05.22 |
申请号 |
US19990269136 |
申请日期 |
1999.07.12 |
申请人 |
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE DEPARTMENT OF HEALTH AND HUMAN SERVICES |
发明人 |
MORRISON CHRISTINE J.;REISS ERROL;HOLLOWAY BRIAN;SHIN JONG HEE |
分类号 |
G01N33/566;C12N15/09;C12Q1/68;G01N33/569;(IPC1-7):C07H21/04;C07H21/02;C12P19/34 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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