PREPARATION OF RECONSTRUCTED FERTILIZED EGG AND PREPARATION OF TRANSGENIC EMBRYO USING THE EGG

摘要

PROBLEM TO BE SOLVED: To provide a process for the preparation of a transgenic reconstructed fertilized egg having totipotency and capable of easily and efficiently expressing a desired gene and a process for producing a transgenic embryo using the egg. SOLUTION: The objective process for the preparation of a reconstructed fertilized egg comprises the injection of a recombinant DNA capable of expressing a desired gene and a reporter gene to enable the detection of the expression in a host cell while keeping the host cell in live state without applying chemical or mechanical treatment on the host cell into the pronucleus of the 1st fertilized egg in the pronucleus stage by micro-injection technique, the selection of a cell expressing the reporter gene and the nuclear transplantation of the nucleus of the selected cell to the 2nd fertilized egg.