Transformation of genes encoding highly alkaline proteases into Bacillus bacteria, and vectors, DNA sequences and probes used in the process

摘要

The invention relates to novel processes and novel industrial Bacillus strains for the increased production of serine protease. Plasmid structures which contain the serine protease gene are introduced into a suitable host which is normally a Bacillus which is already overproducing serine protease. Advantageous host cells are the cells from Bacillus novo strain PB92, and the advantageous serine protease gene is also derived from Bacillus PB92. Integration and maintenance of the plasmid in the host cell chromosome can be achieved by including a temperature-sensitive replication initiation site in the plasmid and by culturing structures under selective conditions.