| 摘要 |
A diagnostic reagent kit comprising a compound of Formula (I) for measuring enzyme activities and metabolites using NADH and NADPH analogs. The analogs have extended stability in aqueous solutions and can used as replacements for NADH or NADPH cofactors in analytical procedures. Disclosed are kits containing the NADH and NADPH analogs for use in the measurement of ALT activity, AST activity, urea, ammonia, salicylate, triglycerides, pyruvic acid, sorbitol dehydrogenase activity, 5'-nucleotidase activity, creatine kinase activity, 2,3-diphosphoglyceric acid, adenosine 5'-triphosphate, alpha -hydroxybutyrate dehydrogenase activity, lactate dehydrogenase activity and the carbon dioxide content in analytical samples. Wherein; R1 is Formula (II); R2 is an aryl or heteroaryl; Q is C or S; T is O or S; X is H, OR3 or H2PO4, where R3 is H, C1-4alkyl, C1-4haloalkyl, C1-4 substituted alkyl or halogen; Y is O, S or NOH; and Z is H, C1-6alkyl, C1-6haloalkyl substituted alkyl, NHL where L is H, OH or NH2, aryl, aralkyl; except that L is not H when R2 is adenine.
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