发明名称 |
Screening for udp-glucose: glycoprotein glucosyltransferase (uggt) activity |
摘要 |
<p>The present invention relates to a method for determining the effect of a test sample on UGGT activity. The method comprises the steps of: a) exposing an acceptor substrate of UGGT such as acid phosphatase to a labeled donor such as UDP-3H-glucose in the presence of the test sample and UGGT; and b) detecting the amount of donor which was transferred to the acceptor substrate wherein a decrease of donor intake when compared to a control means that the test sample is a UGGT stimulator and a decrease means that the test sample is a UGGT inhibitor. The present invention also relates to an isolated mammalian cDNA which encodes for rat UGGT and to methods of producing mammalian UGGT using recombinant vectors.</p> |
申请公布号 |
AU6419700(A) |
申请公布日期 |
2001.03.13 |
申请号 |
AU20000064197 |
申请日期 |
2000.07.27 |
申请人 |
NATIONAL RESEARCH COUNCIL OF CANADA |
发明人 |
DANIEL C. TESSIER;DANIEL DIGNARD;JOHN J. M BERGERON;DAVID Y THOMAS |
分类号 |
C12N15/09;C12N1/15;C12N1/16;C12N1/19;C12N1/21;C12N5/10;C12N9/10;C12N15/54;C12Q1/48;C12R1/865 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|