METHOD FOR ISOLATING AND PURIFYING NATURAL ANTIOXIDANT N-FERULOYLSEROTONIN FROM SAFFLOWER SEEDS ADMINISTRATION)

摘要

PURPOSE: A method for isolating and purifying natural antioxidant N-feruloylserotonin from safflower seeds is provided, thereby the high purity natural antioxidant N-feruloylserotonin is simply and rapidly obtained from safflower seeds. CONSTITUTION: The method for isolating and purifying natural antioxidant N-feruloylserotonin comprises the steps of: grinding safflower seeds and extracting the grinded safflower seeds with methanol; sequentially dividing the extract into ethylacetate, normal butanol and water fractions; dissolving the ethylacetate fraction into organic solvent and passing it through column chromatography to collect several fractions; passing the fractions through TLC and collecting fractions that have the same development distant; and measuring DPPH radical activity of fractions to isolate antioxidant, in which methanol is 70 to 90%, and the extraction is performed at room temperature for 1 day.