发明名称 |
Method for the immunological identification or determination of polypeptide antigens |
摘要 |
<p>Immunoassay for identification or determination of a specific polypeptide antigen X (I) in a sample comprises treating the sample in vitro with≥1 agent (II) which reacts with the epsilon -amino function of a lysine residue or with the alpha -amino residue of a peptide to couple a residue of (II) with (I) and form modified (I), i.e. polypeptide antigen Xm (I'). The immunoassay for the presence of (I) in the sample is then carried out using≥1 antibody (III) specific for (I') but not specific for the modification itself. (III) is produced by immunisation against a polypeptide antigen Y (IV) identical with (I') or its antigenic fragment. Also claimed are:(a) hybridomas producing anti-ACTH monoclonal antibodies acylated by succinic anhydride, deposited at the CNCM on 12.12.95 under the numbers 1-1648, 1-1649 and 1-1650, and(b) the acylated anti-ACTH monoclonal antibodies produced by the hybridomas as in (a).</p> |
申请公布号 |
GR3034962(T3) |
申请公布日期 |
2001.02.28 |
申请号 |
GR20000402665T |
申请日期 |
2000.11.30 |
申请人 |
SOCIETE ANONYME DITE: IMMUNOTECH S.A. |
发明人 |
JEAN, FREDERIC;KERTESZ, GILLES;BOURCIER, BEATRICE |
分类号 |
C07K16/26;C12N5/20;C12P21/08;G01N33/531;G01N33/74;G01N33/78;(IPC1-7):G01N33/531 |
主分类号 |
C07K16/26 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|