METHOD OF ISOLATING SECRETION SIGNALS IN LACTIC ACID BACTERIA AND NOVEL SECRETION SIGNALS ISOLATED FROM LACTOCOCCUS LACTIS

摘要

A method of identifying nucleotide sequences coding for signal peptides in lactic acid bacteria, using a DNA molecule comprising a transposon including a promoterless reporter gene from which DNA molecule a region between the LR a nd the reporter gene is deleted and the DNA molecule comprises a DNA sequence coding for a secretion reporter molecule. By deleting the region between the LR and the reporter gene, stop codons in-frame with the secretion reporter molecule is removed which upon transposition permits translational fusions from upstream the LR.