POLYESTER POLYMERASE AND GENE ENCODING THE SAME

摘要

PROBLEM TO BE SOLVED: To obtain a novel polyester polymerase which comprises a protein containing a specific amino acid sequence and exhibiting polyester polymerase activity and is useful in the production or the like of a polyester that is a soft polymeric material having plasticity as a biodegradable plastic. SOLUTION: This protein is a novel polyester polymerase protein comprising a protein containing the amino acid sequence represented by the formula or a protein which contains an amino acid sequence in which one or several numbers of amino acids are deleted, substituted or added to the amino acid sequence represented by the formula and exhibits polyester polymerase activity and the polymerase is useful in the production or the like of a polyester, e.g. poly-3- hydroxy-alkane acid or the like, which is a soft polymeric material having plasticity as a biodegradable plastic. This protein is obtained by separating a chromosomal DNA from Pseudomonas sp. strain 61-3, preparing a library by a conventional method, screening out the library by a probe comprising the partial base sequence and integrating the resultant gene into an expression system to express.