发明名称 |
Glycosylation variants of iduronate 2-sulfatase |
摘要 |
The present invention provides a highly glycosylated iduronate-2-sulfatase enzyme comprising an iduronate-2-sulfatase polypeptide with at least 5 kilodalton (kDa) more sugar than iduronate-2-sulfatase purified from a natural source, e.g. human liver. The present invention also provides an enzymatically active polypeptide fragment or variant of such a highly glycosylated iduronate-2-sulfatase. The present invention further provides an isolated nucleic acid encoding iduronate-2-sulfatase, as well as an expression vector, a host cell and a method for producing the present highly glycosylated iduronate-2-sulfatase enzyme. In one embodiment the present invention is directed to a method for producing a glycosylated iduronate-2-sulfatase enzyme which comprises culturing a host cell containing a nucleic acid encoding an enzymatically active iduronate-2-sulfatase polypeptide wherein the host cell glycosylates the polypeptide to a greater degree than a native iduronate-2-sulfatase polypeptide expressed by a natural human liver cell.
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申请公布号 |
US6153188(A) |
申请公布日期 |
2000.11.28 |
申请号 |
US19990249003 |
申请日期 |
1999.02.12 |
申请人 |
WOMEN'S AND CHILDREN'S HOSPITAL |
发明人 |
WILSON, PETER J.;MORRIS, CHARLES PHILLIP;ANSON, DONALD STEWART;OCCHIODORO, TERESA;BIELICKI, JULIE;CLEMENTS, PETER ROY;HOPWOOD, JOHN JOSEPH |
分类号 |
A61K38/00;C12N5/18;C12N5/22;C12N9/16;(IPC1-7):A61K38/46 |
主分类号 |
A61K38/00 |
代理机构 |
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地址 |
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