发明名称 |
Process and kit for permeabilizing erythrocytes, and immuno-marking foetal erythrocytes |
摘要 |
Provided is a process for permabilising erythrocytes in vitro characterised in that the erythrocytes are subjected successively to the action of a) a fixing agent containing an aliphatic aldehyde, an oligosaccharide and optionally a sulfated poysaccharide in preferably an isotonic or hypertonic medium with a pH of between 4 and 7; and b) a permeabilising agent containing a detergent and an oligosaccharide preferably with a pH of 5 to 6; and optionally c) after fixation and permeabilisation, the erythrocytes are washed using a washing agent to eliminate the detergent; and optionally the erythrocytes are reacted with two antibodies against two foetal antigens which are expressed independently on the erythrocyte. Also provide is a suitable kit for permeabilising erythrocytes comprising agents a) and b) above.
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申请公布号 |
NZ336635(A) |
申请公布日期 |
2000.11.24 |
申请号 |
NZ19990336635 |
申请日期 |
1999.07.07 |
申请人 |
IMMUNOTECH |
发明人 |
VAN AGTHOVEN, ANDRE;FORNELLI, CHRISTINE |
分类号 |
G01N33/53;G01N33/48;G01N33/50;G01N33/72;G01N33/80;(IPC1-7):A61K35/18;G01N33/555 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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