REAGENT AND PROCESS FOR PEPTIDE/PROTEIN DIFFERENTIATION USING CIRCULAR DICHROISM DETECTION

摘要

A reagent comprised of an aqueous solution of Cu(II)-D-histidine complex acts effectively as a devitalizing agent required to qualitatively identify an enantiomer and quantitatively determine its enantiomeric purities. The initial function of the host ligand (D-histidine) is to keep the Cu(II) ion in solution in high ph values. The base line CD spectrum associated with each Cu-(chiral ligand) host complex is uniquely different. On adding peptide or protein, exchange occurs between the host ligand (D-histidine) in the analyte ligand (protein). Exchanges produce changes in the CD spectrum that are significant enough that they have the potential of becoming a reliable spectroscopic fingerprint for every individual analyte.