| 摘要 |
PROBLEM TO BE SOLVED: To estimate the color of hair for screening an anti-white hair agent and judging the effect of anti-white hair agent, etc., by detecting an mRNA encoding tyrosinase in a separated hair follicle by a method such as RP-PCR. SOLUTION: A scalp tissue is cut out from a surgically ablated scalp right under a basement membrane by a stereomicroscope, a hair follicle in a shape of embedded in a fat tissue is isolated by tweezers, put in a micro-homogenizer, mixed with an RNase inhibitor, ground at a room temperature, an RNA is separated from the ground contents by a routine procedure, treated with an oligo(dT) to isolate a mRNA, which is subjected to an RNA transcription- polymerase chain reaction method (RT-PCR) by using a reverse transcriptase and a pair of primers complementary at two parts of a gene encoding tyrosinase as a PCR primer and an mRNA encoding tyrosinase in the hair follicle is amplified and detected to estimate a hair color. |
