发明名称 |
PHARMACOLOGICAL TARGETING OF mRNA CAP FORMATION |
摘要 |
This invention provides methods for the discovery of molecules that target an essential aspect of eukaryotic gene expression - the formation of the mRNA 5' cap m7GpppN. An underlying principle of this invention is the use of different strains of a test organism that differ only in the composition or source of the essential cap-forming enzymes. The invention provides isogenic yeast strains that derive all their capping activities from fungal sources versus mammalian sources. These strains form the basis of a differential growth inhibition assay to identify molecules that specifically target the fungal capping apparatus. This invention also provides a method to screen in vitro for molecules that inhibit fungal RNA triphosphatase, an essential enzyme that catalyzes the first of three steps in cap synthesis.
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申请公布号 |
WO0063433(A1) |
申请公布日期 |
2000.10.26 |
申请号 |
WO1999US26520 |
申请日期 |
1999.11.09 |
申请人 |
SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCH |
发明人 |
SHUMAN, STEWART |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N9/10;C12N9/12;C12N9/14;C12N15/81;C12Q1/02;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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地址 |
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