| 摘要 |
PURPOSE: A novel SP-PEG-hemoglobin corporate body is provided, which shows safety and effectiveness as an oxygen-carrier, so can be used in medicinal utility. CONSTITUTION: A process for the preparation of SP-PEG-hemoglobin comprises of: pouring methoxy-PEG and sodium ethoxide to a flask, dissolving completely by boiling, and gaining m-PEG ethyl propionate; dissolving the m-PEG ethyl propionate in 1N NaOH, and gaining m-PEG propionic acid; dissolving the m-PEG propionic acid in dichloromethane, adding N-hydroxysuccinate(NHS) and dicyclohexylcarbodiimide in dichloromethane to get m-PEG succinimidyl propionate; separating red blood cells from mammal blood, and separating hemoglobins from the red blood cells; dissolving the hemoglobins in a solution of 0.15M sodium chloride and 0.01M Na-phosphate(pH 8.0), and adding the SP-PEG(hemoglobin:SP-PEG=1:20 as equivalent ratio); reacting at room temperature for 1-2hrs(pH 8.0), and removing un-reacted PEG by ultrafiltration or diafiltration.
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