LIPOPOLYSACCHARIDE IMMUNOASSAY AND TEST DEVICE

摘要

An immunoassay for detecting lipopolysaccharides from Gram negative bacteria such as E.coli, Chlamydia or Salmonella and a device for conducting the same wherein the assay comprises the use of a lipopolysaccharide binding protein and an antibody having specific binding affinity to the lipopolysaccharide analyte as first or second binding reagents. The analyte is reacted with a first binding reagent to form a complex and then reacted with the second binding reagent to form a complex comprising the analyte and first and second binding reagents. The presence of the final complex is then detected. One of the binding reagents is labelled and free, the other binding reagent is unlabelled and immobilised on a solid support. The device comprises a first zone from where the free labelled first binding reagent is carried with the sample to the detection zone comprising the unlabelled second binding reagent via capillary action.