| 摘要 |
<p>A method of determining a substrate, which is contained in various kinds of samples, simply and speedily without requiring a troublesome pretreatment; and a biosensor, the method, in which an electrode system formed by using a conductive material, and a reaction reagent comprising at least a dehydrogenation enzyme, a coenzyme, an electron mediator and tetrazolium salts are used, comprising generating an enzyme reaction and an oxidation reduction reaction by the reaction reagent and a substrate in a sample, and then detecting formazane, a final formed product by using the electrode system; the biosensor utilizing the same method.</p> |
