| 摘要 |
A method for quickly assaying the cAMP content or the adenylate cyclase activity in a biological sample containing endogenous non-cyclic adenine nucleotides without resort to any radioactive reagents. More particularly, a method for assaying the cAMP content or the adenylate cyclase activity in a biological sample containing endogenous non-cyclic adenine nucleotides selected from the group consisting of cAMP formed by endogenous adenylate cyclase, ATP, AMP, ADP and mixtures thereof which comprises: (1) adding efficacious amounts of apyrase, adenosine deaminase and alkaline phosphatase to the above sample to thereby enzymatically remove the endogenous non-cyclic adenine nucleotides other than cAMP and glycose-6-phosphate therefrom; (2) enzymatically converting cAMP into AMP; and (3) thus quantitating AMP without resort to any radioactive substances; and a kit for performing this method.
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