A 3'-end modified nucleic acid probe, suitable for hybridization and detection via fluorescence

摘要

A nucleic acid probe which is a single-stranded nucleic acid complementary to a specific nucleic acid sequence and is labeled so as to give off a measurable fluorescent signal on hybridization with a nucleic acid containing the specific nucleic acid sequence, wherein the 3' end of the probe is modified as represented by the following formula (1): wherein R is -COOH, -CONH2, -(CH2)nOH, -CH(OH)-CH2OH or - CHÄ(CH2)n-NHR1Ü-CH2OH, R1 is H, a dye, or an amino-protecting group such as Fmoc, and n is an integer of at least 1.