| 摘要 |
<p>Resins containing apolar styrene-divinylbenzenc-type matrices may solvate in aqueous medium, depending on the amount of positively charged ammonium groups incorporated in its structure. Experiments with a resin containing 4.9 mmol/g aminomethyl groups and denominated aminomethyl-resin (hereafter referred as to EPM-1) showed that this resin presents a good solvation in polar organic solvents and even in water. Based on a special strategy already developed for estimation of pKa of ionizable groups of the resin, it was found a value around 5,5 for the EPM-1 amine group. As model of anionic solutes for chromatographic assays with EPM-1, differently negatively charged peptide fragments were synthesized and tested in chromatographic purification thus demonstrating that EPM-1 can be considered an alternative anion exchange resin also for other anionic solutes. Besides, for containing easily reactive primary amine groups in its structure, this resin can be also precursor for other resins with different chromatographic potentialities. In the same trend, chemical variation involving the divinylbenzene degree of crosslinking of this copolymer may affect its solvation characteristics, porosity, etc., hence modifying the use of this polymeric material.</p> |
