Verfahren zur Herstellung endotoxinfreier oder an Endotoxin abgereicherter Nukleinsäuren und deren Verwendung

摘要

The invention relates to a method for isolating and purifying nucleic acids and/or oligonucleotides from a biological sample. The invention also relates to the use of the isolated or purified nucleic acid for the transfection of cells and for the production of an agent for treating genetic diseases. The invention further relates to a composition which is suitable for the isolation process or purification process and to the use of potassium acetate and a silica-gel-like base material for isolating endotoxin-free or endotoxin-degraded nucleic acids and/or oligonucleotides.