发明名称 |
METHODS AND REAGENTS FOR MOLECULAR DETECTION OF HIV-1 GROUPS M, N AND O |
摘要 |
Reagents and assays for detecting HIV-1 groups M and O and optionally HIV-1 group N and SIVcpz are provided. The reagents are nucleic acid primers for the hybridization to, amplification and subsequent detection of HIV-1 groups M, N and O and SIVcpz in a biological sample. The primers are oligonucleotides that selectively hybridize to the highly conserved regions of the env and pol regions of HIV-1. The assays employ the primers for HIV-1 nucleic acid amplification using amplification techniques such as reverse transcription and the polymerase chain reaction. The assays are useful for diagnosing an HIV-1 group M, HIV-1 group N, or an HIV-1 group O infection in a patient. Due to the high sensitivity of the assays, small concentrations of HIV in a biological sample can be detected, thereby allowing diagnosis at an early stage of infection. The assays are also useful for detecting HIV-1 contamination in a biological fluid such as blood. The assays are qualitative or quantitative and are therefore useful for viral load determinations of HIV-1 groups M, N or O in a patient undergoing treatment for HIV-1 infection. Viral load determinations can be used to monitor the progress of the treatment regimen or the development of drug resistance and can be used to predict disease progression.
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申请公布号 |
WO0046403(A2) |
申请公布日期 |
2000.08.10 |
申请号 |
WO2000US02498 |
申请日期 |
2000.02.01 |
申请人 |
THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES;LAL, RENU, B.;PIENIAZEK, DANUTA;YANG, CHUNFU |
发明人 |
LAL, RENU, B.;PIENIAZEK, DANUTA;YANG, CHUNFU |
分类号 |
C12Q1/70;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/70 |
代理机构 |
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