Multiplex genotyping of populations of individuals

摘要

This invention provides methods for generating polymorphic profiles for many polymorphic markers in many individuals in a population. The methods involve performing multiplex amplification of the markers in many nucleic acid samples from each of many individuals to produce multiple amplification products. The resulting multiplex amplification products are applied to a substrate to create an array. Then, in one embodiment in a series of iterative passes, pairs of probes that detect both alleles of a marker are hybridized to each amplification product in the array to identify the alleles the individuals have for the marker.