| 摘要 |
The invention relates to a method for electrochemically detecting sequence- specific nucleic acid-oligomer hybridisation events. DNA/RNA/PNA oligomer single strands which are bound to a conductive surface at one end and linked to a redoxactive unit at the other, free end, serve as a hybridisation matri x (probe). A proportion of the single strand oligonucleotides are hybridised b y treatment with the oligonucleotide solution (target) being tested, with the result that the electrical communication between the conductive surface and the redoxactive unit, which is initially non- or barely existent, is increased. This enables a hybridisation event to be detected using electrochemical methods such as voltammetry, amperometry or conductance measurement.
|
