Recombinant expression of proteins from secretory cell lines
摘要
The present invention a provides methods for production of heterologous polypeptides using a variety recombinantly engineered secretory cell lines. The common feature of these cell lines is the absence of expression of at least one endogenous polypeptide. The host cell machinery normally used to produce the endogenous polypeptide is then usurped for the purpose of making the heterologous polypeptide. Also described are methods engineering cells for high level expression, methods of large scale protein production, and methods for treatment of disease in vivo using viral delivery systems and recombinant cell lines.
申请公布号
US6087129(A)
申请公布日期
2000.07.11
申请号
US19960589028
申请日期
1996.01.19
申请人
BETAGENE, INC.;BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
发明人
NEWGARD, CHRISTOPHER B.;NORMINGTON, KARL D.;CLARK, SAMUEL A.;THIGPEN, ANICE E.;QUAADE, CHRISTIAN;KRUSE, FRED