发明名称 |
CHEMICALLY MODIFIED PROTEINS WITH A CARBOHYDRATE MOIETY |
摘要 |
The present invention relates to a chemically modified mutant protein including a cysteine residue substituted for a residue other than cysteine in a precursor protein, the substituted cysteine residue being subsequently modified by reacting the cysteine residue with a glycosylated thiosulfonate. Also, a method of producing the chemically modified mutant protein is provided. The present invention also relates to a glycosylated methanethiosulfonate. Another aspect of the present invention is a method of modifying the functional characteristics of a protein including providing a protein and reacting the protein with a glycosylated methanethiosulfonate reagent under conditions effective to produce a glycoprotein with altered functional characteristics as compared to the protein. In addition, the present invention relates to methods of determining the structure-function relationships of chemically modified mutant proteins.
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申请公布号 |
WO0001712(A9) |
申请公布日期 |
2000.06.22 |
申请号 |
WO1999US15138 |
申请日期 |
1999.07.02 |
申请人 |
GENENCOR INTERNATIONAL, INC. |
发明人 |
JONES, J., BRYAN;DAVIS, BENJAMIN, G. |
分类号 |
C12N15/09;C07K14/00;C12N9/54;C12N9/96;C12Q1/25;C12Q1/37;(IPC1-7):C07K1/107;C12N9/00;C12N15/00;C12N15/52;C12N15/57 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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