Specific, highly sensitive, nested PCR detection scheme for the pseudorabies virus

摘要

The present invention provides for a highly sensitive nested polymerase chain reaction (PCR) method for detecting the presence or absence of the pseudorabies virus (PVR). The method targets a 674 base-pair region of the pseudorabies virus gII gene. Nucleotide sequences for highly specific novel primers derived from this gII region are also disclosed. These primers are used with the nested polymerase chain reaction method to amplify targeted nucleotide sequences within the 674 base-pair region of the gII gene. The novel primers and optimized reaction conditions of the nested polymerase chain reaction method enable significantly greater specificity for the viral DNA in tissue suspected of harboring the latent pseudorabies virus.