| 摘要 |
A method for the production of human naturally secreted extracellular matrix material, comprising: (a) Providing a living stromal tissue (fibroblasts) prepared in vitro comprising human stromal cells and connective tissue proteins naturally selected by the stromal cells attached to and substantially enveloping a framework, said framework composed of a bio-compatible, non-living material formed into a 3-D structure having interstitial spaces bridged by stromal cells (polyamide, polyester, polystyrene, polypropylene, polyacrylate, polyvinyl, polycarbonate, polytetrafluorethylene or nitrocellulose compound); (b) Killing the cells in the living stromal tissue; and (c) Removing the killed cells and any cellular contents from the framework; (d) Collecting the extracellular matrix material deposited on the framework; (e) Processing the collected extracellular matrix material by homogenizing, cross linking, or suspending the extracellular matrix material in a physiological acceptable carrier. The composition of human extracellular matrix components is an injectable preparation, suitable for the treatment and repair of soft tissue and skin defects such as wrinkles and scars.
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