SCREENING FOR ANALYTES USING LABELED RECEPTORS

摘要

A method for assaying an analyte in a sample. The method comprising the steps of a) contacting the sample with material comprising a receptor which is present in a liposome and which liposome comprises a detectable functionality, said contact occurring under conditions resulting in binding of the receptor to analyte if present before or concomitant with step b, wherein step b) consists of contacting the sample with an immobilised ligand for the receptor said contact occurring under conditions resulting in binding of the receptor to the ligand, with steps a and b being followed by c) separating the resulting immobilised ligand-receptor fraction and the receptor fraction present in solution and d) assaying the detectable functionality of the receptor in a fraction from step c) in a manner known per se for its detection. Suitably the receptor is being present in step a) in a concentration between 1 pM - 1 nM and the detectable funcitonality in step a) is present in a concentration of 1 pM - 1 '1M and the immobilised ligand in step b) having a Kd for the receptor <50 nM. The immobilised ligand should be present in an amount required to capture 10-99 % of the receptors in the assay in the absence of analyte at a receptor concentration below the Kd of the immobilised ligand and receptor under conditions otherwise corresponding to those of the assay. The conditions and the detectable functionalities being selected such that a 0.1-10 % change in either the ligand-receptor fraction or in the free receptor fraction can be qualitatively or quantitatively detectable.