SYNTHETIC NUTRIENT MEDIUM FOR BRUCELLA CULTURING

摘要

FIELD: microbiology. SUBSTANCE: nutrient medium has mineral salts, carbon-containing component, amino acids, vitamins at the following ratio of components, g/l: agar-agar 14.0-16.0; glucose 1.0; potassium hydrogen phosphate 7.0; potassium dihydrogen phosphate 2.0; sodium citrate 0.5; magnesium sulfate 0.1; ammonium sulfate 1.0; L-glutamic acid 0.5-1.5; L-cysteine 0.025-0.05; L-lysine 0.025-0.05; L-histidine 0.025-0.05; L-alanine 0.025-0.05; L-methionine 0.025-0.05; thiamine 0.0002; nicotinic acid 0.0002; calcium pantothenate 0.0002; riboflavin 0.0002; pyridoxine 0.0002; biotin 0.0000001 and water up to 1 l. Medium provides nutrient requirements of all known species and biovars of brucella. EFFECT: increased yield of cultured biomass. 1 tbl, 2 ex