MULTIPLE TANDOM INTEGRATION VECTOR FOR HANSENULA POLYMORPHA

摘要

PURPOSE: Provided are an expression vector for Hansenula polymorpha which introduces foreign genes in multiple rows and a method for easily selecting Hansenula polymorpha transformants. CONSTITUTION: An expression vector for Hansenula polymorpha is inserted into cells in multiple rows. At 3'-terminal, the vector has repeated segments of 5'-GGGTGGCG-3', self-replicable segment, promoter, and selective genes for nutritional requirement etc. And a method for selecting Hansenula polymorpha transformants is comprised of the next steps of: i) transforming Hansenula polymorpha with the expression vector; ii) cultivating and selecting transformants in a minimal medium; iii) cultivating the transformants continuously in a complex medium to stabilize vectors; and iv) cultivating stabilized transformants in a selective medium containing antibiotics to select transformants resistant to the antibiotics.