METHODS FOR MANIPULATING COMPLEX NUCLEIC ACID POPULATIONS USING PEPTIDE-LABELED OLIGONUCLEOTIDES

摘要

The present invention relates generally to methods of labeling, sorting and screening populations of nucleic acids. More particularly, the present invention relates to a method for sorting and comparing complex populations of nucleic acid, such as cDNA libraries. These complex populations of nucleic acid may be derived from cells or tissue types having variations in phenotyp e of potential clinical interest. The method is referred to generally as the ValiGeneSM Peptide- Labeled Oligonucleotide method, or VG- PLOSM, and involves the use of distinguishable and identifiable peptide tags linked to identical oligonucleotide primers.