METHOD FOR DETECTING AN ANALYTE AND KIT

摘要

The invention concerns a method for detecting at least an analyte, in a biological fluid sample, which consists in contacting said sample with a ligand, specific at least of one analyte to be detected and fixed on at least a solid support assay zone, marking the bound specific ligand and said at least one analyte, with a tracer, and detecting the tracer as well as contacting said sample with a non-specific ligand, fixed on a zone controlling said solid support threshold and capable of signalling a potential background noise generated over an assay zone by a set of non-specific interacting factors; marking the non-specific ligand and bound interacting factors, with a tracer, and detecting the tracer, the non-specific ligand being fixed on the threshold control zone such that the intensity of the marking signal detected thereon for a biological fluid threshold sample is equal to that of said threshold sample on the assay zone.