Libraries of sense strand cDNA immobilized in the 5'-side. An artificial known base sequence is added to the 3'-side of an antisense cDNA strand (the first strand) and the 5'-side of the second strand is immobilized by using a primer complementary to the above base sequence. Thus, a cDNA library with excellent qualities, which contain the full-length cDNA at a high possibility, can be obtained.
申请公布号
WO0017335(A1)
申请公布日期
2000.03.30
申请号
WO1999JP04549
申请日期
1999.08.24
申请人
HELIX RESEARCH INSTITUTE;OTA, TOSHIO;MITSUHASHI, MASATO;ISOGAI, TAKAO;WAKAMATSU, AI
发明人
OTA, TOSHIO;MITSUHASHI, MASATO;ISOGAI, TAKAO;WAKAMATSU, AI