DNA SEQUENCES ENCODING PHYTASES OF RUMINAL MICROORGANISMS

摘要

PURPOSE: A host cell for representing DNA representing phytases and a method for generating transformed host cell are provided. CONSTITUTION: Phytases derived from ruminal microorganisms are provided. The phytases are capable of catalyzing the release of inorganic phosphorus from phytic acid. Preferred sources of phytases include Selenomonas, Prevotella, Treponemaand Megasphaera. A purified and isolated DNA encoding a phytase of Selenomonas ruminantium JY35 (ATCC 55785) is provided. Recombinant expression vectors containing DNAs encoding the phytases and host cells transformed with DNAs encoding the phytases are also provided. The phytases are useful in a wide range of applications involving the dephosphorylation of phytase, including, among other things, use in animal feed supplements.