| 摘要 |
<p>The invention provides an improved method for quantitation of two target genes in a simultaneous PCR, without the need to determine limiting primer concentrations for both targets. In particular, the invention provides a method of quantitation of expression of a first and a second target protein-encoding nucleic acids using a PCR based assay in which amplification of nucleic acid is detected by release of a reporter fluorescent signal which is measured as a normalised reporter value ('deltaRn').</p> |
