| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new recombinant C-reactive protein(CRP) which is obtained by incorporating a gene which codes for CRP into an expression vector, introducing the obtained vector into a host, transforming the obtained host, followed by culturing the obtained host, and is useful, for example, for the diagnosis, for example, of infection, inflammation, and tissue damage, and clinical examination. SOLUTION: This is a new biologically active recombinant C-reactive protein(CRP) which is obtained by incorporating a gene which codes for CRP into an expression vector, transforming a host cell by the obtained vector, followed by culturing the obtained host cell, allows preparation of CRP antibody which allows precise determination of CRP level in blood which the CRP level in blood is enhanced, for example, by infection, inflammation, and tissue damage, and which determination of the level is useful, for example, for the diagnosis and clinical examination. This recombinant CRP is obtained by amplifying/ cloning by the PCR method using human placenta chromosomal DNA as a template and using primers having a partial gene structure, followed by expressing the obtained gene which codes for CRP as described above. |
