NEUTRAL ENHANCEMENT OF LANTHANIDES FOR TIME RESOLVED FLUORESCENCE

摘要

A method for the spectroscopic determination of a marker comprises: (a) contacting chelated lanthanide metal ions bound to a marker with a buffered solution comprising a detergent, an enhancer reagent and a polyanion, wherein said buffer maintains the pH of the solution within the range of about 3.5 to about 11.5 and said polyanion is present in sufficient concentration such that said lanthanide metal ion disassociates from said chelate complex and reassociates with said enhancer reagent, thereby transferring said lanthanide metal ion into fluorescent form; and (b) determining the amount of lanthanide metal ion liberated from the marker as a measure of the amount of marker present by subjecting the solution to a short radiation pulse and detecting the fluorescence of the lanthanide metal ion after the fluorescence from any background sources substantially has ceased.