Promoter and terminator sequences of formate dehydrogenase gene of Candida boidinii

摘要

PCT No. PCT/JP96/02597 Sec. 371 Date May 9, 1997 Sec. 102(e) Date May 9, 1997 PCT Filed Sep. 12, 1996 PCT Pub. No. WO97/10345 PCT Pub. Date Mar. 20, 1997A promoter for a formate dehydrogenase gene from Candida boidinii, substantially comprising a 190 bp or more continuous nucleotide sequence selected from the nucleotide sequence of SEQ ID NO:1; a promoter for a formate dehydrogenase gene from Candida boidinii, substantially comprising the nucleotide sequence of SEQ ID NO:1, 48, 49 or 50; a terminator for a formate dehydrogenase gene from Candida boidinii, substantially comprising the nucleotide sequence of SEQ ID NO:2; a gene expression cassette comprising said promoter, a heterologous gene and said terminator; a recombinant expression vector comprising said gene expression cassette; a transformant transformed with said recombinant expression vector; a process for producing an expression product of a heterologous gene, which comprises culturing said transformant and recovering an expression product of a heterologous gene from the culture.