发明名称 |
Method for transformation of cotton and kenaf and organogenic regeneration |
摘要 |
Protocols for ogranogenic regeneration of cotton and kenaf are provided, which makes the in vitro regeneration of mature fertile plants in a reduced amount of time possible. Seedlings are the basis for monocotyl or hypocotyl explants which are transferred from the germination medium to a shoot initiation medium which comprises AgNO3. These explants, prior to shoot initiation, may be transformed with exogenous DNA either through inoculation with a Agrobacterium agent such as A. tumefaciens, or through biolistic bombardment of the explants with microprojectiles having the exogenous DNA adsorbed onto their surface.
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申请公布号 |
US5998207(A) |
申请公布日期 |
1999.12.07 |
申请号 |
US19980096360 |
申请日期 |
1998.06.12 |
申请人 |
MISSISSIPPI STATE UNIVERSITY |
发明人 |
REICHERT, NANCY A.;LIM, TEONG-KWEE;YOUNG, MARGARET M. |
分类号 |
A01H4/00;C12N15/82;(IPC1-7):A01H4/00;A01H1/00;C12N15/84 |
主分类号 |
A01H4/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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