| 摘要 |
<p>A method of detecting the presence or absence of the 713-8delC sequence variation in the human TGF- beta 1 gene is described, which method comprises provision of a sample comprising the TGF- beta 1 gene to be tested, or a relevant fragment thereof, subjecting the sample to a PCR amplification process using as upstream primer an oligonucleotide comprising a 15-100 nucleotides long section selected coherently from the following nucleotide sequence: <IMAGE> such as to comprise the bold A* (the A marked with an asterisk) nucleotide, and as downstream primer a 15-100 nucleotides long oligonucleotide selected such as to correspond to a coherent sequence section of the TGF- beta 1 gene, placed at least one nucleotide upstream the 713-8C or 713-8delC, respectively, position of the gene (cf. Figs. 1 and 2a,b of the drawings), then digesting the PCR amplification product(s) thus produced with a restriction enzyme recognizing and cleaving the following nucleotide sequence: <IMAGE> subjecting the digested product(s) to a restriction fragment length separation process and detecting whether a restriction fragment length corresponding to a cleavage of the PCR amplification product(s) is present or not. <IMAGE></p> |
