PHOSPHOGLYCERATE KINASE GENE PROMOTER, ITS TERMINATOR OR/AND GENE EXPRESSION UNIT COMPOSED OF THE SAME, GENOME GENE OF PHOSPHOGLYCERATE KINASE AND CDNA GENE

摘要

PURPOSE:To provide the subject promoter derived from genome gene of phosphoglycerate kinase of Aspergillus oryze, capable of mass-producing phosphoglycerate kinase and capable of efficiently producing various kinds of useful substances. CONSTITUTION:Aspergillus oryze R11340 strain is seeded in a culture medium, cultured at 30 deg.C for 3 day, subsequently collected, ground, suspended in a buffer solution, treated with phenol-chloroform and subjected to ethanol precipitation to isolate chromosome DNA. The isolated chromosome DNA is treated with a restriction enzyme and the resultant respective fragments are bonded to a vector to form a gene library of Aspergillus oryze chromosome DNA. The obtained gene library is subsequently screened by using a partial base sequence of phosphoglycerate kinase gene as a probe to select positive clones. From the selected positive clones, the plasmid is recovered and treated with a restriction enzyme, thus obtaining the objective phosphoglycerate kinase gene promoter.